Volume 5 Issue 2
Jun.  2012
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Article Navigation >  Water Science and Engineering  > 2012  >  5(2): 145-154
Yong JI, Guang-hua LU, Chao WANG, Jie ZHANG. 2012: Biochemical responses of freshwater fish Carassius auratus to polycyclic aromatic hydrocarbons and pesticides. Water Science and Engineering, 5(2): 145-154. doi: 10.3882/j.issn.1674-2370.2012.02.003
Citation: Yong JI, Guang-hua LU, Chao WANG, Jie ZHANG. 2012: Biochemical responses of freshwater fish Carassius auratus to polycyclic aromatic hydrocarbons and pesticides. Water Science and Engineering, 5(2): 145-154. doi: 10.3882/j.issn.1674-2370.2012.02.003
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Biochemical responses of freshwater fish Carassius auratus to polycyclic aromatic hydrocarbons and pesticides

doi: 10.3882/j.issn.1674-2370.2012.02.003
  • 1.

    College of Environment, Hohai University, Nanjing 210098, P. R. China 

  • 2.

    School of Hydraulic and Ecological Engineering, Nanchang Institute of Technology, Nanchang 330099, P. R. China

Funds:  the Special Fund for Public Welfare Industry of Ministry of Water Resources of China (Grant No. 201001056), the Project of Jiangxi Provincial Department of Science and Technology (Grant No. 2010BSA20300), and the Project of Jiangxi Provincial Department of Education (Grants No. GJJ11246 and GJJ11636).
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  • Corresponding author: Yong JI
  • Received Date: 2011-04-20
  • Rev Recd Date: 2011-10-26
    Abstract
  • The freshwater fish Carassius auratus was chosen as an experimental subject, and their hepatic biochemical responses to the medium-term exposure of Benzo(k)fluoranthene (BkF) alone and in combination with PCB118 and dichlorodiphenyltrichloroethane (DDT) were investigated by measuring the reduced glutathione (GSH), glutathione S-transferase (GST), and thiobarbituric acid reactive substances (TBARS), to assess sublethal effects. The hepatic GSH content was significantly inhibited by organic pollutants, alone and in mixtures, while the TBARS content was significantly induced after three days of exposure. Bell-shaped concentration-response charts of GST activities were obtained. Significant dose-response relationships were found for hepatic GSH and TBARS contents of all concentrations and for the GST activity, except at the highest concentration. The GSH content, GST activity, and TBARS content in Carassius auratus were confirmed as useful biomarkers of exposure to organic pollutions.

     

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