Water Science and Engineering 2019, 12(3) 213-220 DOI:   https://doi.org/10.1016/j.wse.2019.09.003  ISSN: 1674-2370 CN: 32-1785/TV

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Acid mine drainage
Iron-oxidizing bacteria
Biooxidation of ferrous iron
Hydraulic retention time
Influent pH
Microbial community analyses

Effect of hydraulic retention time and pH on oxidation of ferrous iron in simulated ferruginous acid mine drainage treatment with inoculation of iron-oxidizing bacteria

Jun-hui Fan a, Xing-yu Liu a,*, Qi-yuan Gu a, Ming-jiang Zhang a, Xue-wu Hu a,b

a GRIMAT Engineering Institute Co., Ltd., Beijing 101407, China
b School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing 100083, China 


The effect of hydraulic retention time (HRT) and pH on the biooxidation of ferrous iron during simulated acid mine drainage (AMD) treatment was investigated. The simulated AMD was highly acidic (pH 2.5), rich in iron (about 1700 mg/L) and copper (about 200 mg/L), and contained high concentrations of sulfate (about 4700 mg/L). The biooxidation of ferrous iron was studied in a laboratory-scale upflow packed bed bioreactor (PBR). The HRT was shortened stepwise from 40 h to 20 h, 13 h, and 8 h under the acidic environment at a pH value of 2.2. Then, the influent pH value was changed from 2.2 to 1.2 at a constant suitable HRT. Physiochemical and microbial community structure analyses were performed on water samples and stuffing collected from the bioreactor under different conditions. The results indicate that the efficiency of ferrous iron oxidation gradually decreased with the decrease of HRT, and when the HRT exceeded 13 h, ferrous iron in AMD was almost completely oxidized. In addition, the best efficiency of ferrous iron oxidation was achieved at the influent pH value of 1.8. Microbial community structure analyses show that Leptospirillum is the predominant genus attached in the bioreactor, and low influent pH values are suitable for the growth of Leptospirillum.

Keywords Acid mine drainage   Iron-oxidizing bacteria   Biooxidation of ferrous iron   Hydraulic retention time   Influent pH   Microbial community analyses  
Received 2018-11-26 Revised 2019-05-02 Online: 2019-09-30 
DOI: https://doi.org/10.1016/j.wse.2019.09.003

This work was supported by the National Natural Science Foundation of China (Grant No. U1402234); the Guangxi Scientific Research and Technology Development Plan (Grants No. GuikeAB16380287 and GuikeAB17129025); the Public Welfare Fund of the Ministry of Environmental Protection of China (Grant No. 201509049); the Program of International S & T Cooperation (Grant No. 2016YFE0130700); and the Fund of the General Research Institute for Nonferrous Metal (Grants No. 53321 and 53348).

Corresponding Authors: Xing-yu Liu
Email: wellwoodliu@163.com
About author:


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